Bioinformatics Analysis of Bushen Anzhi Recipe for Aging-Related Insomnia

342 insomnia and 213 control samples from the GSE98582 dataset underpin this analysis of a Traditional Chinese Medicine formula. The study identifies 25 effective components and 16 neuroinflammation and drug target-related differentially expressed genes. Haiming Li published this 20.6 KB Excel file on figshare in May 2026 under a CC-BY-4.0 license.

Use Cases

• Identify potential drug targets for insomnia treatments based on the 16 neuroinflammation and drug target-related differentially expressed genes.
• Analyze multi-level interaction networks for herbal compounds based on the core target genes HIF1A, PTGS2, and GSK3B mentioned in the results.
• Investigate the association between the Wnt signaling pathway and insomnia treatment based on the changes in β-catenin and Wnt3a levels reported.
• Validate bioinformatics findings with experimental data based on the described rat model and polysomnography results.

Strengths

• Data is derived from a substantial human sample set of 555 total samples (342 insomnia, 213 control).
• Analysis is supported by experimental validation in a rat model with 60 subjects across three groups.
• The dataset is openly available under a permissive CC-BY-4.0 license.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The dataset is very small at 20.6 KB, indicating it likely contains summary or analysis results rather than raw expression data.
• Freshness should be verified as the last update is dated 2026-05-08, which is in the future relative to the current date.

Provenance

Source

Gene Expression Omnibus (GEO) dataset GSE98582, Traditional Chinese Medicine Systems Pharmacology and HERB databases.

Collection Method

Bioinformatics analysis of gene expression data, network and enrichment analyses, combined with experimental validation in a rat model.

Time Range

The underlying GEO dataset GSE98582 likely has its own temporal coverage, which is not specified here.

Freshness

Last updated 2026-05-08 05:54:41; freshness should be verified.

Geography

The human sample geography is not specified; the experimental validation was conducted on Sprague Dawley rats.