Six Core Gene Markers for Colorectal Cancer Diagnosis

Six core genes (EIF2S3, GTF3A, HMGA1, HSP90AB1, PABPC1, S100A11) were identified as malignant transformation markers in colorectal polyps. The diagnostic model was validated using the TCGA-COADREAD cohort and external dataset GSE41258. Author Tao He published the findings in March 2026.

Use Cases

• Validate the six-gene diagnostic model (EIF2S3, GTF3A, HMGA1, HSP90AB1, PABPC1, S100A11) against new patient RNA-seq data.
• Assess the correlation between high expression of EIF2S3 and S100A11 with patient prognosis using survival analysis.
• Compare mRNA expression levels of the six core genes between CRC cell lines (SW480, HCT116) and normal epithelial cell line NCM460.
• Investigate protein expression levels of HMGA1 and HSP90AB1 in tumor tissues using the UALCAN database.

Strengths

• Model validation includes an external independent cohort (GSE41258) for performance verification.
• Six specific core genes are identified and listed, providing clear biomarkers for analysis.
• Findings are supported by multiple analytical methods including WGCNA, Boruta algorithm, LASSO regression, and XGBoost.

Limitations

• The dataset is very small at 35.7 KB, indicating limited raw data or a summary table rather than a primary research dataset.
• Unknown row and column counts prevent assessment of the dataset's scale and feature completeness.
• Data is derived from specific public cohorts (GSE209741, GSE161277, TCGA-COADREAD), which may limit generalizability to other populations.

Provenance

Source

Analysis derived from public datasets GSE209741, GSE161277, and the TCGA-COADREAD cohort.

Collection Method

Bioinformatics analysis using edgeR, WGCNA, Boruta algorithm, LASSO regression, XGBoost, and ridge regression modeling.

Freshness

Last updated March 2026.