Bioinformatics Insights on Rapamycin and CaMK2D in Intestinal Ischemia-Reperfusion Injury

Ruxiang Sheng published a research document on figshare in 2026 detailing bioinformatic and experimental analyses of rapamycin's therapeutic mechanisms in intestinal ischemia-reperfusion injury. The study includes RNA-Seq transcriptomic profiling, molecular docking studies, and results from cellular and murine models. The dataset is a 2.0 MB DOCX file licensed under CC-BY-4.0.

Use Cases

• Analyze differential gene expression patterns based on RNA-Seq data mentioned in the description
• Study protein interaction network clustering based on the MCODE analysis described
• Investigate molecular docking results between rapamycin and CaMK2D based on the RAPA-CaMK2D docking studies
• Examine correlations between CaMK2D phosphorylation levels and inflammatory markers based on the described western blot and ELISA results

Strengths

• Includes results from both cellular (Caco-2 OGD/R model) and animal (murine I/R model) experiments, providing a multi-model validation.
• Bioinformatic analyses are detailed, including RNA-Seq, differential expression, and protein interaction network clustering.
• Specific experimental results are reported, such as rapamycin dosage (1.5 mg/kg) and statistical significance (P < 0.01, P < 0.05).

Limitations

• The dataset is a single 2.0 MB DOCX document; the underlying raw data files (e.g., RNA-Seq reads) are not directly accessible.
• Column-level documentation is absent; field semantics must be inferred from the research article text.
• Row count is unknown, which may limit suitability assessment for direct computational analysis.

Provenance

Source

Ruxiang Sheng via figshare

Collection Method

Experimental research involving RNA sequencing, bioinformatic analysis, cellular assays, and animal models.

Freshness

Last updated 2026-05-01 04:19:05; freshness should be verified.