Zebrafish BMPR2 Mutant Cardiac Phenotype and Transcriptomic Data

Zebrafish data from a study on BMPR2 receptor subtypes bmpr2a and bmpr2b in cardiac valve development. It contains transcriptomic profiling and cardiac function assessment results from three CRISPR/Cas9-generated mutant strains. The dataset includes measurements of valve-related genes and cardiac markers at 52 hours post-fertilization.

Use Cases

• Analyze expression levels of cardiac markers like myl9a, tnnc1a, and myl7 across bmpr2a-/- and bmpr2b-/- mutant strains.
• Correlate valve-related gene expression (e.g., fn1b, has2, nfatc1) with observed cardiac contractile dysfunction phenotypes.
• Investigate the downregulation of klf2a in relation to the ECM-receptor interaction pathway identified in the study.
• Compare transcriptomic profiles between single knockout (bmpr2a-/- or bmpr2b-/-) and double knockout (bmpr2a-/-;bmpr2b-/-) zebrafish.

Strengths

• Data is derived from three distinct, CRISPR/Cas9-generated zebrafish mutant strains (bmpr2a-/-, bmpr2b-/-, bmpr2a-/-;bmpr2b-/-).
• Includes multi-modal validation via RNA-seq, whole-mount in situ hybridization (WISH), and qRT-PCR.
• Focuses on a specific developmental time point of 52 hours post-fertilization for phenotype characterization.

Limitations

• The dataset is small at 84.9 KB, indicating limited scope and likely a summary table rather than raw sequencing data.
• Specific row and column counts are unknown, limiting assessment of its analytical depth.
• Data is restricted to a single model organism (zebrafish) and a specific developmental stage.

Provenance

Source

figshare, authored by Yan Shi.

Collection Method

Experimental data from CRISPR/Cas9 mutagenesis, cardiac function assessment (M-mode), and transcriptomic profiling (RNA-seq).

Time Range

Observations at 52 hours post-fertilization.

Freshness

Last updated March 2026.