Han-Def1 and Han-Def2: Characterization of Novel Tick Defensins

Two novel defensin genes, Han-Def1 and Han-Def2, were identified from the tick Hyalomma anatolicum. The dataset contains bioinformatic and experimental characterization results, including antimicrobial activity, hemolysis, and antioxidant capacity. The data was authored by Tingxiang Luo and shared on figshare under a CC-BY-4.0 license, last updated on 2026-05-14.

Use Cases

• Identify novel antimicrobial peptide candidates based on the bioinformatic analysis of tick defensins.
• Assess peptide biocompatibility for therapeutic development based on the reported low hemolytic rate (<2%).
• Compare antimicrobial spectra based on the described activity against Staphylococcus aureus, Enterococcus faecalis, and Bacillus subtilis.
• Study structure-activity relationships in defensins based on the described conserved cysteine-stabilized α/β fold.

Strengths

• Experimental results include specific efficacy metrics, such as a hemolytic rate of <2% at 750 μg/mL.
• The description provides concrete antimicrobial activity against three specific Gram-positive bacteria.
• Data is shared under a permissive CC-BY-4.0 license.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is a 6.7 MB DOCX file, which may require parsing to extract structured data.

Provenance

Source

figshare

Collection Method

Genes were cloned, recombinantly expressed, and assessed for antimicrobial, hemolytic, and antioxidant activities.

Freshness

Last updated 2026-05-14 05:23:31; freshness should be verified.