BxGST3 and BxGST1 Gene Expression Data for Pine Wood Nematode

Bo Nian cloned and analyzed two glutathione S-transferase genes, BxGST3 and BxGST1, in the pine wood nematode Bursaphelenchus xylophilus. The dataset likely contains quantitative PCR results showing up-regulation during infection, with peak expression at 24 hours post-inoculation. The data was uploaded to figshare on 2026-05-25.

Use Cases

• Analyze gene expression patterns during host infection based on quantitative PCR results
• Study gene function in nematode physiology based on RNA interference silencing effects
• Investigate localization of gene expression in nematode tissues based on in situ hybridization findings

Strengths

• Includes specific fold-change expression data (5.1-fold for BxGST3, 3.1-fold for BxGST1)
• Describes functional analysis methods including cloning, qPCR, RNAi, and in situ hybridization
• Available under an open CC-BY-4.0 license

Limitations

• Dataset size is 14.9 KB, indicating a very limited scope
• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment

Provenance

Source

figshare

Collection Method

Cloning and functional analysis via RACE, qPCR, in situ hybridization, and RNAi.

Freshness

Last updated 2026-05-25 06:03:49