Novel Spectinomycin Resistance Gene Characterization Data

Data from a 2026 study by Susu Chen characterizing a novel plasmid-encoded antibiotic resistance gene, ant(9)-If. It includes enzyme kinetic parameters, minimum inhibitory concentration (MIC) fold changes, and sequence analysis results. The gene was discovered in Enterococcus faecium isolated from animal farm sewage.

Use Cases

• Analyze the relationship between ant(9)-If gene presence and spectinomycin MIC fold-change values.
• Compare enzyme kinetic parameters (Km, kcat/Km) of ANT(9)-If with other aminoglycoside adenylyltransferases.
• Study the distribution of ant(9)-If-like genes across plasmids and chromosomes in Gram-positive bacteria using sequence analysis data.
• Model the affinity and catalytic efficiency of ANT(9)-If for spectinomycin using the provided Michaelis constant and kcat/Km values.

Strengths

• Includes precise enzyme kinetic measurements: Michaelis constant (Km) of 4.599 ± 0.198 μM.
• Reports a 32-fold increase in spectinomycin MIC for the recombinant strain.
• Provides amino acid identity comparison (64.70%) to known resistance enzyme ANT(9)-Ia.

Limitations

• Dataset is very small at 28.0 KB, indicating limited scope and likely a single table of summary results.
• Contains data from a single bacterial isolate (Enterococcus faecium 520), limiting generalizability.
• Sample origin is specific to animal farm sewage, which may not represent clinical or other environmental sources.

Provenance

Source

Figshare, authored by Susu Chen.

Collection Method

Data generated via whole-genome sequencing, agar dilution MIC tests, molecular cloning, protein expression, and bioinformatic analysis.

Time Range

Study published in 2026.

Freshness

Last updated in April 2026.

Geography

Sample isolated from an animal farm sewage source; specific location not provided.