Bovine Sperm Proteomic Profiles Before and After Cryopreservation

A proteomic dataset characterizing changes in bovine sperm proteins due to cryopreservation. The study identified 1,373 proteins across samples from five bulls, with 84 showing significant differential abundance. The dataset was created by Alexandra Keller and last updated on 2026-04 16.

Use Cases

• Train classification models to predict sperm cryosurvival based on the 84 differentially abundant proteins.
• Perform functional enrichment analysis to map protein changes to pathways like metabolism and cytoskeletal organization.
• Validate potential biomarkers such as CYCT, DDOST, and RPS3A for cryoinjury using the provided proteomic profiles.

Strengths

• Includes results from five sexually mature bulls with proven fertility, providing a relevant biological cohort.
• Identifies 1,373 proteins and 84 with significant differential abundance, offering a substantive molecular profile.
• Uses a bottom-up proteomic approach with LC-MS/MS analysis, a standard methodology in the field.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small at 158.4 KB, indicating a limited scope focused on summary results rather than raw spectra.

Provenance

Source

figshare, author Alexandra Keller.

Collection Method

Bottom-up proteomic approach using LC-MS/MS on semen samples divided into fresh and cryopreserved groups.

Time Range

The temporal coverage of the underlying study is not specified.

Freshness

Last updated 2026-04-16 05:26:13; freshness should be verified.

Geography

The spatial coverage of the underlying study is not specified.