TRIM28-MAVS Ubiquitination Data from Antiviral Signaling Study

119.9 KB Excel file contains experimental results from a study on TRIM28's role in suppressing antiviral immunity. The dataset includes measurements from TRIM28 knockdown/overexpression systems, protein interaction assays, and ubiquitination site analysis on MAVS. Author Ruihua Xin published the data in 2026 under a CC BY 4.0 license.

Use Cases

• Analyze the correlation between TRIM28 knockdown levels and type I IFN response measurements from in vitro assays.
• Examine protein interaction data from co-immunoprecipitation assays between TRIM28 and MAVS.
• Map the functional impact of MAVS lysine mutants (e.g., Lys136, Lys461) on downstream signaling metrics like TBK1 activation.
• Compare virus replication inhibition data from in vitro experiments with results from the AAV-mediated lung-specific TRIM28 knockdown model.

Strengths

• Data is directly tied to a 2026 publication, ensuring recent scientific relevance.
• Includes results from multiple experimental methods: confocal microscopy for trafficking, co-immunoprecipitation, and in-cell ubiquitination assays.
• Explicitly identifies specific MAVS ubiquitination sites (Lys136, Lys461) with functional validation.

Limitations

• The dataset is very small at 119.9 KB, indicating limited scope, likely containing summary results rather than raw high-throughput data.
• Unknown row and column structure makes direct computational analysis challenging without manual inspection.
• Data is specific to a single mechanistic study on the TRIM28-MAVS axis, limiting generalizability to other antiviral pathways.

Provenance

Source

Ruihua Xin, published on figshare.

Collection Method

Experimental data from molecular biology techniques including TRIM28 knockdown/overexpression, confocal microscopy, co-immunoprecipitation, and ubiquitination assays.

Freshness

Last updated March 2026.