Urine Proteome in Boys with Posterior Urethral Valves, Pilot Cohort

20 boys with posterior urethral valves (PUV) and 20 unaffected controls were analyzed using liquid chromatography-tandem mass spectrometry to define the urine proteome. The study, authored by Xin Wang and last updated in May 2026, developed eight complementary workflows for differential expression analysis and assembled a single-cell RNA-seq atlas of 162,083 healthy kidney, ureter, and bladder cells to infer tissue origins.

Use Cases

• Identify protein biomarkers for kidney injury progression based on estimated glomerular filtration rate (eGFR) variation.
• Investigate urothelial injury and remodeling pathways based on upregulated keratins and uroplakins.
• Map differential protein expression to specific renal and urothelial cell types using the provided single-cell RNA-seq atlas.
• Explore associations between complement activation, inflammation proteins, and declining renal function.

Strengths

• Dataset includes a matched control group of 20 unaffected individuals.
• Analysis integrates proteomic data with a single-cell RNA-seq atlas of 162,083 cells for biological context.
• Eight complementary analytical workflows were developed to ensure robust detection of differential protein expression.

Limitations

• Row and column counts are unknown, limiting suitability assessment for specific analytical needs.
• Column-level documentation is absent; field semantics must be inferred after download.
• The pilot cohort of 20 cases is small, as noted in the study description.

Provenance

Source

figshare

Collection Method

Liquid chromatography-tandem mass spectrometry proteomic analysis of urine samples.

Freshness

Last updated 2026-05-13 05:56:56; freshness should be verified.