Horizontal Gene Transfer Events in Euplotes Ciliate Genomes

Ruanlin Wang's research data details the detection and analysis of 342 horizontal gene transfer (HGT) candidates across five sequenced Euplotes genomes. The dataset includes functional annotations from Pfam, GO, and KEGG databases, as well as validation results from qPCR and RNAi experiments for mannan endo-1,4-β-mannosidase genes. This supporting information for the associated research article was uploaded to figshare in April 2026.

Use Cases

• Analyzing the functional distribution of the 342 HGT-acquired genes using their Pfam, GO, and KEGG annotations.
• Comparing genomic features like GC content, intron proportion, and CDS length between HGT-acquired and native genes.
• Investigating the seven HGT families putatively transferred into the last common ancestor of all five Euplotes species.
• Studying the five putative endosymbiont-derived glycoside hydrolase genes identified in E. vannus.
• Validating gene essentiality hypotheses using the qPCR expression data and RNAi mortality results for Ea-Man genes.

Strengths

• Contains a curated list of 342 HGT candidate genes with systematic functional annotation.
• Includes experimental validation data (qPCR, RNAi) for key gene functions.
• Data is directly linked to a peer-reviewed research methodology and analysis.

Limitations

• The dataset is very small (13.6 KB), contained within a single DOCX file, limiting machine-readability.
• The underlying raw genomic and transcriptomic sequence data is not included, only the analysis results.
• Scope is limited to five specific Euplotes species, reducing generalizability to other organisms.

Provenance

Source

Ruanlin Wang, research associated with a study on HGT in Euplotes.

Collection Method

Phylogeny-based HGT detection performed on five genome-sequenced Euplotes, followed by gene structure, expression, and functional annotation analysis.

Time Range

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Freshness

Last updated in April 2026.

Geography

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