TaqMan RT-qPCR Assay Validation for Six Tick-Borne Viruses in Northeastern China

Shiyao Xu developed and validated single-plex TaqMan real-time quantitative PCR assays for detecting six tick-borne pathogenic viruses in northeastern China. The dataset, last updated on 2026-06-04, includes results from validation trials comparing the assays against established reference methods using field-collected tick samples. The assays target Alongshan virus (ALSV), Tick-borne encephalitis virus (TBEV), Severe fever with thrombocytopenia syndrome virus (SFTSV), Beiji nairovirus (BJNV), Yezo virus (YEZV), and Songling virus (SGLV).

Use Cases

• Validate diagnostic assays for tick-borne viruses based on sensitivity and specificity results.
• Compare detection methods for low viral load samples based on the performance differences between TaqMan and SYBR Green RT-qPCR.
• Support epidemiological surveillance of tick-borne viruses in northeastern China based on field-collected sample data.

Strengths

• Assays demonstrated a limit of detection as low as 10 copies/μL for all six target viruses.
• Specificity testing confirmed no cross-reactivity among the six viral targets.
• Validation showed complete concordance with reference methods for ALSV (5/15) and TBEV (4/14).

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small at 28.6 KB, indicating limited scope.

Provenance

Source

Shiyao Xu

Collection Method

Primer and probe sets were designed using Beacon Designer 8.0, with sensitivity assessed using recombinant plasmids and specificity verified using virus-positive cDNA from tick samples.

Freshness

Last updated 2026-06-04 05:42:51; freshness should be verified.

Geography

Northeastern China