Duplex Crystal Digital PCR Assay for PRRSV-1 and PRRSV-2 Detection in Clinical Samples

Guangxi Province in South China is the source for 2,185 clinical tissue samples used to validate a novel diagnostic method. The dataset, authored by Yuwen Shi and last updated in March 2026, contains results from a duplex crystal digital PCR assay developed for the differential detection of two genotypes of the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). It includes performance metrics like sensitivity, specificity, and positivity rates for PRRSV-1 and PRRSV-2.

Use Cases

• Benchmarking new diagnostic assays based on the reported sensitivity and specificity metrics.
• Studying PRRSV genotype prevalence based on the positivity rates for PRRSV-1 and PRRSV-2.
• Developing machine learning models for veterinary diagnostics based on PCR assay performance data.

Strengths

• Assay validation includes 2,185 clinical samples, providing a substantial test base.
• Reported limits of detection are 4.507 and 5.607 copies/reaction for PRRSV-1 and PRRSV-2, respectively.
• High coincidence rates (over 99.7%) with established qPCR methods are documented.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect geographic bias inherent to the collection from Guangxi Province.

Provenance

Source

Yuwen Shi via figshare.

Collection Method

Clinical testing of tissue samples using a developed duplex crystal digital PCR assay.

Freshness

Last updated 2026-03-18 07:35:06; freshness should be verified.

Geography

Guangxi Province, South China.