ant(9)-Ie: Novel Spectinomycin Resistance Gene in Providencia Rettgeri

A 17.5 KB research document describes the discovery and characterization of a novel chromosomally encoded aminoglycoside resistance gene, ant(9)-Ie, in Providencia rettgeri P17. The gene, identified from wastewater on an animal farm, confers a 64-fold increase in spectinomycin MIC and has a catalytic efficiency of (8.22 ± 1.24) × 10² M⁻¹·s⁻¹. The document, authored by Lulu Huang and last updated in June 2026, includes results from molecular cloning, kinetic analysis, and comparative genomics.

Use Cases

• Comparative genomic analysis of antimicrobial resistance genes based on the described conserved genetic environment of ant(9)-Ie.
• Kinetic modeling of enzyme activity based on the reported spectinomycin catalytic efficiency (kcat/Km).
• Phylogenetic analysis of resistance gene backgrounds based on the amino acid sequence similarity to ANT(9)-Id (58.67%).
• Studying the epidemiology of resistance genes in Providencia strains based on the mention of isolation from human clinical specimens.

Strengths

• Includes specific kinetic parameter for the novel enzyme: spectinomycin catalytic efficiency of (8.22 ± 1.24) × 10² M⁻¹·s⁻¹.
• Reports a concrete 64-fold increase in the Minimum Inhibitory Concentration (MIC) of spectinomycin for the recombinant strain.
• Provides a clear comparative figure: ANT(9)-Ie has 58.67% amino acid sequence similarity to ANT(9)-Id.

Limitations

• The primary data (e.g., sequence files, raw MIC values) are not directly accessible; the dataset is a 17.5 KB DOCX summary document.
• Row count and column-level documentation are absent; data semantics must be inferred from the narrative.
• The dataset's scale is very limited (17.5 KB), containing a single study's summarized findings.

Provenance

Source

Lulu Huang, via figshare.

Collection Method

Bacteria isolated from animal farm wastewater; gene function elucidated via molecular cloning, heterologous expression, and kinetic analysis; genomic characteristics analyzed via whole-genome sequencing.

Freshness

Last updated 2026-06-02 04:28:44.