Anti-CD28 VHHs for Trispecific T Cell Engagers: Discovery and Characterization

34 VHHs (single-domain antibodies) isolated from a phage-display library generated using PBMCs of a Bactrian camel immunized with recombinant human CD28 protein. The VHHs bound to cell surface CD28 with EC50 values ranging from 8.5 nM to 130 nM and were incorporated into trispecific T cell engagers targeting DLL3, CD3, and CD28. The dataset, authored by Hui Wang and last updated on 2026-04-17, describes the discovery, functional ranking, and structural mapping of these co-stimulatory modules.

Use Cases

• Ranking co-stimulatory activity of anti-CD28 VHHs based on EC50 binding values ranging from 8.5 nM to 130 nM
• Evaluating tumor-specific cytotoxicity of trispecific T cell engagers compared to bispecific engagers
• Structural mapping of VHH epitopes based on residues Arg34, Tyr51, and Tyr61 identified through alanine-scanning mutagenesis
• Selecting functional modules for trispecific T cell engagers based on integrated affinity screening and therapeutic efficacy evaluation

Strengths

• Contains binding affinity data for 34 distinct VHHs
• Includes structural mapping and mutagenesis results for a key candidate (VHH38)
• Provides comparative efficacy data between trispecific and bispecific T cell engagers

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• The 1.5 MB size suggests the dataset is primarily descriptive text rather than structured numerical data

Provenance

Source

figshare

Collection Method

Data likely originates from a laboratory study involving phage-display library screening and functional assays.

Freshness

Last updated 2026-04-17 05:22:13; freshness should be verified