SIRT3 Interaction and Cardiac Mitochondrial Response to Hypoxia-Reoxygenation

A 2026 study by Joshua W. Kranrod investigates the cardioprotective metabolite SA-22 and its direct interaction with the mitochondrial deacetylase SIRT3. The research, shared on figshare, includes experimental results on ligand binding, catalytic activity, and SIRT3-dependent protection against hypoxia-reoxygenation injury in H9c2 cardiac cells. The dataset is a 2.3 MB document detailing methodology and findings.

Use Cases

• Study the molecular mechanism of SIRT3 allosteric modulation based on the described thermal shift and mutagenesis assays.
• Model cardiac cell response to hypoxia-reoxygenation injury based on described mitophagy and mitochondrial respiration measurements.
• Investigate the cardioprotective role of CYP450-derived lipid metabolites like 19,20-EDP based on the described experimental framework.

Strengths

• Includes specific experimental details such as treatment concentrations (e.g., 1 µM SA-22, 30 µM NAM).
• Describes a clear methodological sequence involving in vitro assays and cell-based models.
• Released under a permissive CC-BY-4.0 license.

Limitations

• The primary data file is a 2.3 MB DOCX document; underlying raw numerical or image data is not explicitly provided.
• Column-level documentation is absent; field semantics must be inferred from the document text.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.

Provenance

Source

Joshua W. Kranrod via figshare.

Collection Method

Experimental research involving thermal shift assays, fluorogenic substrate assays, point mutagenesis, and cell culture models.

Freshness

Last updated 2026-06-02 04:28:35.