Mouse Model of Thermal-Induced Spinal Cord Injury with Transcriptomic Analysis

A figshare document by Arata Mashima, last updated March 17, 2026, describes an experimental mouse model for thermal-induced spinal cord injury. The model involves applying 90°C heat for 1 minute to the thoracic spinal cord, resulting in motor deficits, demyelination, and collagen deposition. Transcriptomic analysis via RNA-sequencing identified upregulated pro-fibrotic genes and implicated specific pathways in scar progression.

Use Cases

• Study the pathophysiology of thermal damage in spinal surgery based on the described mouse model.
• Analyze gene expression changes related to fibrosis based on the mentioned RNA-sequencing results for Col1a1, Col1a2, Tgfβ1, and Acta2.
• Investigate the temporal progression of fibrotic scar formation based on observations at 7 and 14 days post-injury.
• Explore signaling pathways involved in spinal cord injury repair based on the KEGG analysis highlighting extracellular matrix organization and Wnt/β-catenin signaling.

Strengths

• Document includes specific experimental parameters (90°C for 1 minute).
• Findings are supported by multiple analysis methods: behavioral assessment, histology, and RNA-sequencing.
• The dataset is openly available under a CC-BY-4.0 license.

Limitations

• The primary data (e.g., raw RNA-seq data, quantitative measurements) are not directly included in the 19.1 KB document.
• Row count and column structure for any underlying data are unknown.
• The dataset's scale is limited to the described experimental study.

Provenance

Source

Arata Mashima via figshare.

Collection Method

Experimental study involving controlled thermal exposure to mouse spinal cords, followed by behavioral, histological, and transcriptomic analysis.

Freshness

Last updated 2026-03-17 17:12:55.