Exosomal POSTN in Lung Adenocarcinoma: Single-Cell and Exosome Sequencing Data

Jie Chen's research dataset on figshare, last updated 2026-05-19, investigates the role of periostin (POSTN) in cancer-associated fibroblast-derived exosomes for microinvasive lung adenocarcinoma progression. The dataset integrates single-cell RNA sequencing from four primary MIA lesions with matched adjacent tissues, tissue-derived exosomal RNA sequencing, and analyses from the TCGA-LUAD cohort. It includes functional co-culture and xenograft assay results to validate POSTN's role in promoting tumor cell proliferation and invasion.

Use Cases

• Identify cell-cell communication networks in the tumor microenvironment based on single-cell RNA sequencing data mentioned in the description
• Discover prognostic biomarkers for lung adenocarcinoma based on exosomal POSTN expression levels correlated with survival
• Validate the functional role of cancer-associated fibroblast-derived exosomes based on co-culture and xenograft assay results
• Analyze differential gene expression in tissue-derived exosomes based on the 588 differentially expressed mRNAs identified

Strengths

• Integrates multiple data modalities: single-cell RNA-seq, exosomal RNA-seq, and TCGA cohort analysis
• Includes functional validation data from co-culture experiments and xenograft assays
• Focuses on a clinically relevant early-stage cancer (microinvasive adenocarcinoma)

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Data is contained in a 7.6 MB DOCX file, which may not be a standard format for structured bioinformatics analysis

Provenance

Source

figshare, author Jie Chen

Collection Method

Single-cell RNA sequencing and tissue-derived exosomal RNA sequencing performed on four primary MIA lesions and matched adjacent lung tissues, integrated with public TCGA-LUAD data.

Freshness

Last updated 2026-05-19 05:28:32; freshness should be verified