Flow Cytometry Data for Rapid Yeast Detection in Spiked Blood Cultures

A novel flow-cytometric method detects yeast cells in blood cultures in just over 1 hour, compared to days for culture-based methods. The study investigated the performance of two fluorescent markers across 6 different yeast species in spiked blood cultures and validated the gating strategy using imaging cytometry. The limit of detection was 2.871 × 10³ cells/mL in spiked cultures, and the assay successfully detected yeast in clinical blood cultures.

Use Cases

• Benchmarking rapid diagnostic assays based on the described flow-cytometric method and limit of detection
• Training machine learning models for cell classification based on flow cytometry data from spiked cultures
• Studying staining specificity for yeast versus non-yeast cells using the described fluorescent markers

Strengths

• Limit of detection is quantified at 2.871 × 10³ cells/mL for spiked cultures.
• Method validation includes imaging cytometry and confocal microscopy.
• Assay was tested on clinical isolates across 6 different yeast species.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data is derived from a study using spiked cultures and a specific clinical validation set.

Provenance

Source

Malgorzata K. Kopczyk

Collection Method

Experimental data from a study developing a flow-cytometric assay using spiked blood cultures and clinical samples.

Freshness

Last updated 2026-04-23 05:34:37; freshness should be verified.