Bighead Carp MAPK Family Characterization from Genome to Single-Cell Splenic Response

15 MAPK genes were identified in bighead carp and characterized across 24 species. Single-cell RNA sequencing of 28,348 spleen cells revealed 13 distinct cell types and the upregulation of mapk6 and mapk11 during Aeromonas hydrophila infection. The dataset, authored by Yang Mao and last updated in April 2026, provides genomic and transcriptomic data supporting immune response analysis.

Use Cases

• Comparative genomics analysis of MAPK family evolution based on the survey of 382 sequences across 24 species.
• Single-cell transcriptomic profiling of immune cell populations based on the 28,348 spleen cells sequenced.
• Weighted gene co-expression network analysis (WGCNA) to identify infection-related gene modules.
• Pseudotime trajectory analysis of myeloid cell activation during bacterial challenge.
• Cell-cell communication analysis to infer signaling interactions like tnf-tnfrsf1b during immune response.

Strengths

• Includes single-cell RNA sequencing data from 28,348 spleen cells, identifying 13 distinct cell types.
• Integrates multiple analysis methods: comparative genomics, WGCNA, qRT-PCR, and pseudotime analysis.
• Provides high-confidence identification of 15 MAPK genes in bighead carp, classified into ERK, JNK, and p38 subfamilies.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The primary data is presented in a DOCX document, which may require extraction and conversion for computational analysis.

Provenance

Source

figshare

Collection Method

Comparative genomic survey, tissue expression profiling, transcriptome sequencing, qRT-PCR, WGCNA, and single-cell RNA sequencing.

Time Range

Study period not specified; dataset reflects a specific experimental challenge.

Freshness

Last updated 2026-04-13 04:35:38; freshness should be verified.

Geography

Focus on bighead carp (Hypophthalmichthys nobilis); geographic origin of samples not specified.