Pseudomonas shiyinii ST4: Genomic and Functional Characterization for Biocontrol

A 2026 study by Yonglin Li presents the complete 5.14-Mb genome sequence of Pseudomonas sp. ST4, a biocontrol agent against sugarcane smut. The research includes genomic, phylogenetic, and chemotaxonomic analyses, plus a Tn5 transposon mutagenesis screen identifying the PQQ-Gcd pathway as essential for antifungal activity. The dataset is available as a 2.8 MB DOCX file under a CC-BY-4.0 license.

Use Cases

• Identify genes associated with plant-beneficial traits based on the described biosynthetic gene clusters.
• Study the conservation of biocontrol mechanisms like the PQQ-Gcd system across Pseudomonadaceae based on the phylogenomic analysis.
• Investigate the role of acidification in inhibiting fungal pathogen mating based on the described primary mechanism.
• Explore the biosynthesis pathways for phytohormones like indole-3-acetic acid (IAA) and their derivatives as described.

Strengths

• Complete genome sequence of 5.14 Mb for a novel bacterial species.
• Functional validation via Tn5 mutagenesis screen, with 78% of loss-of-function mutants mapping to specific genes.
• Phylogenomic analysis contextualizing traits across the Pseudomonadaceae family.

Limitations

• Data is presented in a DOCX document; structured data files (e.g., FASTA, GFF) are not specified.
• Row and column counts are unknown, limiting assessment of data scale and structure.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

Yonglin Li, via figshare.

Collection Method

Genomic sequencing, phylogenetic analysis, and transposon mutagenesis screen as described.

Freshness

Last updated 2026-05-20 14:53:42; freshness should be verified.