Genomic Determinants of Antifungal Activity in Streptomyces Strain STM-2

Surfacing genomic analysis results for Streptomyces melanosporofaciens STM-2, a strain isolated from Chiayi County, Taiwan. The analysis identified 52 biosynthetic gene clusters (BGCs) and specific genes for antifungal enzymes like chitinases and proteases. It includes results from physiological characterization, genome mining, and comparative pangenomic analysis.

Use Cases

• Analyze the distribution of 52 predicted BGCs, including type I/II PKS and NRPS systems, to identify potential novel antimicrobial compounds.
• Examine genes for antifungal hydrolytic enzymes like chitinases and β-1,3-glucanases to model mechanisms of fungal cell wall degradation.
• Use the high-resolution ANI and dDDH scores from comparative pangenomic analysis to assess the evolutionary uniqueness of the STM-2 strain.
• Investigate the accessory genome containing unique genes to understand specialized antifungal activity for biocontrol applications.

Strengths

• Identifies 52 biosynthetic gene clusters (BGCs) predicted by antiSMASH, providing a detailed genetic inventory.
• Includes high-confidence structural evidence from AlphaFold2 protein models with very high pLDDT scores.
• Integrates multiple analysis methods: physiological characterization, genome mining, comparative genomics, and evolutionary analysis.

Limitations

• The dataset is very small at 19.7 KB, indicating limited scope and likely a summary table rather than raw genomic sequences.
• Rows and columns are unknown, preventing assessment of data volume and feature granularity.
• Data is derived from a single novel strain (STM-2), limiting generalizability to other Streptomyces species.

Provenance

Source

figshare, authored by Muhammed Opoku Gyamfi.

Collection Method

Integrated approach involving physiological/biochemical characterization, genome sequencing/annotation, antiSMASH mining, comparative pangenomics, and AlphaFold2 structural modeling.

Freshness

Last updated March 20, 2026.

Geography

Streptomyces melanosporofaciens STM-2 strain isolated from Chiayi County, Taiwan.