High-Throughput miRNA Screen Against Intracellular Staphylococcus aureus

A 2026 high-throughput screening dataset from figshare contains results from testing 2,469 human miRNA mimics in A549 epithelial cells infected with S. aureus USA300. The data identifies candidate miRNAs like miR-4430, miR-147a, and miR-1249-5p that reduced intracellular bacterial burden and modulated host pathways. The dataset, provided by Pablo Castañera, is shared under a CC BY 4.0 license.

Use Cases

• Identify miRNA mimics from the 2,469 tested that correlate with reduced intracellular S. aureus burden for candidate prioritization.
• Analyze transcriptomic host response data linked to validated miRNAs like miR-4430 to map immune pathway activation.
• Compare the effects of miR-1249-5p and miR-147a across different S. aureus strains and epithelial cell lines for validation studies.
• Perform network centrality analysis on screening results to find multifunctional antimicrobial miRNA candidates.

Strengths

• Screens a large, defined library of 2,469 human miRNA mimics for systematic discovery.
• Validation includes multiple S. aureus strains and epithelial cell lines, increasing result robustness.
• Includes complementary transcriptomic (RNA-seq) profiling data to examine host pathway responses.
• Data is recent, last updated in March 2026, and shared under a permissive CC BY 4.0 license.

Limitations

• The dataset is small (261.8 KB), indicating limited raw data or summarized results rather than full experimental outputs.
• Specific column structure, row count, and sample data are unavailable, hindering immediate analytical use.
• Findings are specific to the A549 epithelial cell model and S. aureus USA300 strain, limiting generalizability to other cell types or pathogens.

Provenance

Source

figshare, authored by Pablo Castañera.

Collection Method

High-throughput screen of miRNA mimics in infected cells, followed by network analysis and RNA-seq validation.

Time Range

Experiment date unspecified; dataset published in 2026.

Freshness

Last updated 2026-03-25.