Human Biliary Atresia Organoid Transcriptomic and Functional Data

Adi Har-Zahav generated patient-derived extrahepatic cholangiocyte organoids from bile duct remnants of biliary atresia patients and controls. The dataset likely contains transcriptomic profiling and functional validation data, including measurements of ER stress markers, xenobiotic metabolism, and cell polarity genes. The data was last updated in May 2026 and is available as a 746.9 KB Excel file.

Use Cases

• Identify dysregulated ER stress pathways based on transcriptomic profiling and elevated PERK, BiP, and ATF4 markers.
• Analyze alterations in xenobiotics and drug metabolism based on CYP450 inhibition experiments.
• Study changes in cell adhesion and polarity based on altered expression of E-cadherin, RhoU, SOX17, and CFTR.
• Assess susceptibility to toxic injury based on functional validation of organoids.
• Validate findings in tissue samples based on immunostaining of liver hilum biopsies confirming elevated markers.

Strengths

• Data is derived from patient-specific organoids, providing a disease-relevant model.
• Includes both discovery bulk RNA-seq and targeted molecular and functional validation.
• Immunostaining of patient liver biopsies provides tissue-level confirmation.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small (746.9 KB), indicating limited scope.

Provenance

Source

figshare

Collection Method

Organoids generated from patient bile duct remnants, followed by RNA-seq and molecular/functional assays.

Freshness

Last updated 2026-05-07 04:37:37; freshness should be verified.