Providencia huaxiensis PR66: Whole-Genome Sequence with Novel NDM-1 ICE

A single clinical Providencia huaxiensis isolate, PR66, was found to harbor a novel 127,074 bp integrative and conjugative element (ICE) carrying the blaNDM-1 carbapenemase gene. The dataset includes the whole-genome sequence analysis of the strain's chromosome and three plasmids, identifying multiple resistance genes. The data was authored by Jia Tao and shared on figshare under a CC-BY-4.0 license in May 2026.

Use Cases

• Analyzing the genetic structure of a novel SXT/R391 integrative and conjugative element based on the 127,074 bp ICE sequence
• Studying the co-location and transfer potential of multiple antibiotic resistance genes, including blaNDM-1, blaOXA-4, and blaOXA-10
• Investigating the site-specific integration of mobile genetic elements into bacterial chromosomes based on the prfC locus integration site
• Comparative genomics of carbapenem-resistant Providencia species based on the whole-genome sequence of the PR66 isolate

Strengths

• The ICE element is precisely characterized with a defined length of 127,074 bp and a GC content of 48.6%.
• Conjugation and genetic stability assays confirmed the successful transfer and stable inheritance of the blaNDM-1 gene.
• Species identification was confirmed using average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH).
• The dataset documents a rarely reported NDM-producing Providencia huaxiensis isolate.

Limitations

• The dataset is based on a single bacterial isolate (PR66), limiting population-level inferences.
• Column-level documentation for any associated sequence files is absent; field semantics must be inferred after download.
• The primary data file is a 1.2 MB DOCX document, which may not contain the raw sequence data in standard bioinformatics formats.

Provenance

Source

Jia Tao

Collection Method

Whole-genome sequencing and bioinformatics analysis of a clinical bacterial isolate.

Freshness

Last updated 2026-05-22 06:10:42