In Vitro RORγt+ Regulatory T Cell Protocol with Functional and Metabolic Data

An optimized protocol increased the frequency of RORγt+ regulatory T cells in vitro by up to 70%. The document details methods for generating these cells, comparing their suppressive function to conventional iTreg, and analyzing their OXPHOS-dependent metabolism. Marcella Cipelli authored this 15.2 KB document, last updated on 2026-05-21.

Use Cases

• Replicating the in vitro differentiation protocol for RORγt+ Treg cells based on the described methodology.
• Comparing immunosuppressive activity between RORγt+ Treg and conventional iTreg cells based on functional assays mentioned.
• Studying the link between oxidative phosphorylation (OXPHOS) and Treg cell function based on the metabolic profile analysis.
• Developing potential therapeutic strategies for immune-mediated diseases based on the enhanced immunosuppressive activity described.

Strengths

• The protocol achieved a specific, quantifiable outcome, increasing RORγt+ Treg cell frequency by up to 70%.
• The description provides concrete details on functional comparison (suppressive activity) and metabolic analysis (OXPHOS reliance).
• The dataset is shared under a permissive CC-BY-4.0 license, facilitating reuse.

Limitations

• The dataset is a 15.2 KB DOCX document, indicating a very limited scope focused on a protocol rather than a large-scale data collection.
• Row count and column-level documentation are unknown, which limits suitability assessment for computational analysis.
• Description metadata is limited; actual data quality and structure require manual inspection after download.

Provenance

Source

figshare

Collection Method

In vitro experimental protocol development and evaluation.

Freshness

Last updated 2026-05-21 05:35:01; freshness should be verified.