Table 1_Inhibition of GPR68: Ferroptosis in Diffuse Intrinsic Pontine Glioma Cells

A 7.5 MB Excel file contains experimental data on the inhibition of GPR68 in pediatric brain cancer cells. Uploaded by Leif R. Neitzel to figshare in May 2026, the data likely includes cell viability, glutathione levels, and gene expression measurements. It details the sensitivity of three DIPG cell lines and normal human astrocytes to the inhibitor Ogremorphin.

Use Cases

• Compare drug sensitivity (LD50) between DIPG cell lines and normal human astrocytes based on CellTiter-Glo viability assays.
• Analyze ferroptosis markers like glutathione levels and lipid peroxidation in response to GPR68 inhibition.
• Investigate the role of ATF4 and SLC7A11 gene expression in ferroptotic cell death based on qRT-PCR data.
• Validate genetic knockdown and overexpression experiments on GPR68 and ATF4 pathways.

Strengths

• Includes data from three distinct DIPG cell lines (SF7761, DIPG36, DIPG007) and normal human astrocytes for comparison.
• Reports specific LD50 values for drug sensitivity (e.g., NHAs: 3.84 µM, DIPG cells: ≤ 0.79 µM).
• Covers multiple experimental validation methods, including rescue experiments and genetic knockdown/overexpression.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect experimental bias inherent to in vitro cell line studies.

Provenance

Source

figshare

Collection Method

Experimental cell biology assays including viability, qRT-PCR, and genetic manipulation.

Freshness

Last updated 2026-05-13 04:48:24; freshness should be verified.