Ocimum Sanctum Nanoemulsion Bioactivity Data from Metabolomics and Pharmacology Study

Featuring experimental results from a study developing and characterizing an Ocimum sanctum (Holy Basil) nanoemulsion. It includes physicochemical characterization data, cytotoxicity results against four cancer cell lines, and bioactivity measurements for antibacterial, antioxidant, and anti-inflammatory effects. The data supports the integrated metabolomic and network pharmacology approach described in the research.

Use Cases

• Analyze the relationship between nanoemulsion droplet size, polydispersity index (PDI), and zeta potential with cytotoxicity IC50 values across Caco-2, HepG2, MDA-MB-231, and A549 cell lines.
• Model the biphasic release profile data to predict compound delivery kinetics over the eight-day measurement period.
• Correlate antioxidant activity (SC50 values) with antibacterial inhibition zone measurements to identify multifunctional bioactivity patterns.
• Use network pharmacology-predicted anti-inflammatory targets (e.g., AKT1, STAT3, PTGS2, TLR4) to contextualize experimental TNF-α and IL-6 secretion data from LPS-stimulated RAW 264.7 macrophages.

Strengths

• Includes multi-faceted bioactivity data covering cytotoxicity, antibacterial, antioxidant, and anti-inflammatory assays.
• Reports specific physicochemical metrics for the nanoemulsion, including droplet size (51–73 nm), PDI (0.264), and zeta potential (-42.1 mV).
• Provides comparative potency data between the nanoemulsion and crude extract, with IC50 values ranging from 13–25 μg/mL for cancer cells.
• Released under a permissive CC BY 4.0 license, facilitating reuse and analysis.

Limitations

• The dataset is small in file size (161.0 KB), indicating limited scope and likely a summary of key results rather than raw experimental data.
• As a preliminary in vitro study, the findings require validation through more extensive in vitro and in vivo investigations as noted by the authors.
• The specific structure, column names, and row count of the underlying tabular data are unknown from the provided description.

Provenance

Source

figshare, authored by Mervat S. Mohamed.

Collection Method

Data generated from laboratory experiments including GC–MS and LC–QTOF–MS/MS metabolomic profiling, nanoemulsion preparation and characterization, and in vitro biological assays.

Freshness

Last updated in March 2026, indicating recent data.