SNRPA Overexpression RNA-seq and iRIP-seq Analysis in HepG2 Cells

498 differentially expressed genes and 2,316 alternative splicing events were identified in HepG2 cells overexpressing the RNA-binding protein SNRPA. This dataset, created by Qingyao Chang and last updated in May 2026, integrates RNA-seq and iRIP-seq data to link SNRPA binding to transcriptomic and splicing alterations relevant to liver cancer.

Use Cases

• Identify SNRPA-regulated transcripts and alternative splicing events based on RNA-seq data.
• Map SNRPA RNA interactomes based on iRIP-seq binding data.
• Analyze the overlap between SNRPA-bound RNAs and genes with altered expression or splicing.
• Investigate the regulatory network of RNA-binding proteins in hepatocellular carcinoma based on the 12 identified RBPs.

Strengths

• Identifies 498 differentially expressed genes (DEGs) and 2,316 alternative splicing events (RASEs).
• Integrates two complementary sequencing methods (RNA-seq and iRIP-seq).
• Data is openly shared under a CC-BY-4.0 license.

Limitations

• Row count and column-level documentation are unknown, requiring manual inspection after download.
• The dataset is small (1.6 MB), suggesting it may contain summary results rather than raw sequencing data.
• Direct causal effects noted in the description require further functional validation.

Provenance

Source

figshare

Collection Method

Generated from SNRPA-overexpressing HepG2 cells, with RNA-seq and iRIP-seq performed and integrated via bioinformatics.

Freshness

Last updated 2026-05-01 04:17:47; freshness should be verified.