Irisin and β-Amyloid-Induced Microglial Inflammation: Molecular Analysis

A research document detailing an in vitro study on the anti-inflammatory effects of the myokine irisin on human microglia stimulated by β-amyloid. The work was authored by Roberta Mancuso and last updated in April 2026. The 746.9 KB document likely contains experimental results and discussion on microRNA and inflammasome pathways.

Use Cases

• Analyzing molecular pathways linking irisin to reduced neuroinflammation based on the described TLR4/NLRP3 axis.
• Studying microRNA expression patterns (miR-451a, miR-223-3p, miR-7-5p) in activated microglia based on the experimental methods.
• Investigating in vitro models of β-amyloid-induced microglial activation based on the described cell culture and stimulation protocols.
• Reviewing methodological approaches for quantifying inflammasome components (e.g., digital droplet PCR, flow cytometry) based on the described techniques.

Strengths

• Document is licensed under CC-BY-4.0, permitting open sharing and adaptation.
• Description provides a detailed account of experimental aims, methods, and molecular targets.
• Last update timestamp (2026-04-23) is provided, indicating recent maintenance.

Limitations

• Row count and column-level documentation are absent; data structure must be inferred from the text.
• The 746.9 KB file size suggests a limited scope, likely containing a single research document rather than a large dataset.
• Data is confined to an in vitro model; its relevance to in vivo conditions requires further investigation as noted in the description.

Provenance

Source

figshare

Collection Method

In vitro laboratory study using human immortalized microglia (hTERT) with LPS priming and Aβ1-42 stimulation.

Time Range

Study period not specified; document updated in 2026.

Freshness

Last updated 2026-04-23 04:19:13

Geography

Geographic coverage not specified.