In Vitro Gut-Brain Barrier Function Data for Lacticaseibacillus rhamnosus HN001

A dataset from figshare, authored by Dulantha Ulluwishewa and last updated in May 2026, presents results from a two-stage in vitro gut-brain axis model. It likely contains measurements of transepithelial electrical resistance and cytokine levels from Caco-2 and hCMEC/D3 cell monolayers. The data supports the investigation of how the probiotic strain HN001 influences intestinal and blood-brain barrier integrity.

Use Cases

• Modeling probiotic effects on intestinal barrier function based on transepithelial electrical resistance data.
• Investigating blood-brain barrier protection mechanisms using data from endothelial monolayer co-culture experiments.
• Analyzing transcriptional modulation of tight-junction proteins like OCLN and CLDN2 mentioned in the description.
• Studying the independent regulation of barrier function from specific cytokines (IL-6, IL-8, MCP-1, TNF-α, IL-1β).

Strengths

• Data is derived from a controlled two-stage in vitro model, allowing for mechanistic investigation.
• The description details specific biological markers and experimental conditions, such as aerobic and apical-anaerobic co-culture.
• Dataset is openly available under a CC-BY-4.0 license.

Limitations

• The dataset is very small at 65.9 KB, indicating limited scope.
• Row count and column-level documentation are absent; field semantics must be inferred after download.
• Data is from an in vitro model, which may not fully translate to in vivo biological systems.

Provenance

Source

figshare

Collection Method

Data likely originates from laboratory experiments using differentiated Caco-2 and hCMEC/D3 cell monolayers in a co-culture model.

Freshness

Last updated 2026-05-08 05:56:57; freshness should be verified.