Sertoli Cell Maturation Data from Frozen-Thawed Mouse Testicular Tissue Cultures

Laura Moutard's dataset contains measurements from an experiment on Sertoli cell maturation in frozen-thawed prepubertal mouse testicular tissue cultured in vitro. The data includes results from immunohistochemistry, RT-qPCR, and ELISA analyses performed on explants cultured for 16 and 30 days, compared to in vivo controls. It was last updated on 2026-04-23 and is shared under a CC-BY-4.0 license.

Use Cases

• Analyzing changes in Sertoli cell number and marker expression over time based on culture duration mentioned in the description
• Comparing in vitro maturation outcomes to in vivo controls based on the described experimental design
• Investigating the impact of FSH supplementation on spermatogenesis progression based on the described culture condition
• Evaluating Sertoli cell functionality through inhibin B levels as described in the experiment

Strengths

• Data is derived from a controlled experiment with specific culture durations of 16 and 30 days.
• Analysis includes multiple techniques: immunohistochemistry, RT-qPCR, and ELISA.
• Includes comparison to in vivo control tissues from postpartum mice.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small at 11.9 KB, indicating limited scope.

Provenance

Source

Laura Moutard via figshare

Collection Method

Experimental data from in vitro culture of frozen-thawed testicular fragments from 6-day-old prepubertal mice, with subsequent immunohistochemical, RT-qPCR, and ELISA analyses.

Freshness

Last updated 2026-04-23 05:34:50; freshness should be verified.