Taq DNA Polymerase Activity in TaqMan qPCR: Engineered Variant Performance

A novel screening assay quantified the polymerase and nuclease activities of Taq DNA polymerase during quantitative PCR. The dataset, created by Qiuyu Cao and last updated in May 2026, includes analysis of engineered Taq variants and KlenTaq, revealing a synergistic relationship between extension and probe cleavage. Findings identify variants with improved cleavage efficiency for multiplex detection and KlenTaq's enhanced strand displacement for specialized applications.

Use Cases

• Optimizing multiplex qPCR assay design based on quantified probe cleavage efficiency.
• Selecting polymerase variants for specialized applications like probe melting curve analysis based on strand displacement activity.
• Benchmarking engineered enzyme performance against wild-type Taq and KlenTaq based on integrated extension and cleavage readouts.
• Informing the design of novel polymerases based on the mechanistic interplay between synthesis and nuclease activities.

Strengths

• Data is derived from a systematic quantification of enzyme activities using a novel integrated assay.
• Analysis includes specific engineered Taq variants and the KlenTaq construct, allowing for comparative study.
• Dataset is openly available under a CC-BY-4.0 license.

Limitations

• The dataset is very small at 15.0 KB, indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

Qiuyu Cao via figshare

Collection Method

Systematic quantification via a novel screening assay integrating extension and cleavage readouts.

Freshness

Last updated 2026-05-20 05:56:29; freshness should be verified.