Neovaginal Microbiota Structure and Source Contributions After Vaginoplasty

A study of 30 transgender women assigned male at birth characterizes the early neovaginal microbiota following penile inversion vaginoplasty. The dataset includes 16S rRNA sequencing and aerobic culture results from penile, scrotal, urethral, rectal, and neovaginal sites, collected from 2 to 13 months post-surgery. It was authored by Lili von Bargen and last updated in May 2026.

Use Cases

• Identify core microbial genera in the neovagina based on the described presence in over 50% of samples.
• Analyze source contributions to the neovaginal microbiota based on shared Amplicon Sequence Variants (ASVs) with donor sites.
• Compare bacterial richness and diversity between the neovagina, gut, and other tissue sites.
• Investigate implications for probiotic therapy development based on the described colonization patterns.

Strengths

• Includes data from 30 participants, providing a basis for cohort-level analysis.
• Combines 16S rRNA sequencing with complementary aerobic culturing results.
• Tracks microbial samples from multiple body sites over a defined post-operative period of 2 to 13 months.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.

Provenance

Source

figshare

Collection Method

16S rRNA sequencing and aerobic culture of samples from pre- and post-operative sites.

Time Range

Samples collected from 2 to 13 months after surgery.

Freshness

Last updated 2026-05-13 05:49:21; freshness should be verified.