MicroRNA Differential Expression in Canine Visceral Hemangiosarcoma FFPE Tissues

A 2026 study by Laura Machado Ribas analyzed formalin-fixed, paraffin-embedded tissues from 24 dogs with visceral hemangiosarcoma and 12 non-neoplastic controls. The dataset contains results from small RNA sequencing and RT-qPCR validation, identifying 67 and 71 differentially expressed miRNAs in splenic and cardiac tumors, respectively. Pathway enrichment analysis linked validated miRNAs to oncogenic signaling pathways.

Use Cases

• Identify potential miRNA biomarkers for canine visceral hemangiosarcoma based on differential expression analysis.
• Validate sequencing results with RT-qPCR data for candidate miRNAs mentioned in the study.
• Perform pathway enrichment analysis based on validated miRNAs to explore oncogenic signaling involvement.
• Compare miRNA signatures between splenic and cardiac tumor types as described in the results.

Strengths

• Data is derived from a controlled study with 24 tumor and 12 control samples.
• Results include validation by RT-qPCR for 40 candidate miRNAs, showing strong concordance with sequencing.
• Pathway analysis reveals significant involvement in specific oncogenic pathways (PI3K-Akt, MAPK, HIF-1, Ras).

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small at 12.5 KB, indicating limited scope.

Provenance

Source

figshare, author Laura Machado Ribas

Collection Method

Small RNA sequencing and quantitative PCR analysis of FFPE tissues.

Time Range

Study likely conducted prior to 2026 publication date.

Freshness

Last updated 2026-03-17 14:40:02; freshness should be verified.

Geography

null