Modeling Ischemic-Type Biliary Lesion Using Human Intrahepatic Cholangiocyte Organoids

A 20.2 KB document by Zirong Liu, last updated in May 2026, describes the construction of an in vitro model for ischemic-type biliary lesion (ITBL). The model uses human intrahepatic cholangiocyte organoids (ICOs) cultured under ischemia and hypoxia conditions. The study evaluates the model's validity using immunofluorescence, RT-qPCR, Western blotting, and transcriptomic analysis.

Use Cases

• Studying the effects of ischemia and hypoxia on cholangiocyte organoids based on the described experimental model.
• Investigating inflammatory and apoptotic responses in biliary tissue based on the described transcriptomic analysis.
• Validating in vitro models of post-liver transplantation complications based on the described methodology for ITBL.

Strengths

• Document is shared under a permissive CC-BY-4.0 license.
• Description provides specific experimental methods and a clear research objective.

Limitations

• The dataset is a 20.2 KB document; the underlying experimental data (e.g., transcriptomic results) are not directly included.
• Row count and column-level documentation are unknown, limiting suitability assessment for direct data analysis.

Provenance

Source

Zirong Liu via figshare.

Collection Method

Likely contains research methods and results from culturing human intrahepatic cholangiocyte organoids under controlled conditions.

Freshness

Last updated 2026-05-29 06:07:23.