Mycoplasma Bovis Infection Alters Extracellular Vesicle Protein Cargo from Bovine Cells

193 proteins demonstrated significantly different abundance in small extracellular vesicles from bovine endometrial epithelial cells infected with Mycoplasma bovis. The dataset was generated using a novel three-dimensional in vitro cell culture model with static bioreactors and analyzed via liquid chromatography-trapped ion mobility spectrometry-tandem mass spectrometry. Joel T. Pratt published the proteomic analysis results under a CC-BY-4.0 license in May 2026.

Use Cases

• Identify novel protein biomarkers for Mycoplasma bovis infection based on the 193 differentially abundant proteins.
• Study host-pathogen interactions by analyzing changes to host Histone proteins and Mycoplasma-derived metabolic enzymes.
• Validate three-dimensional in vitro cell culture models for studying extracellular vesicle biology in bacterial infections.

Strengths

• Proteomic analysis identified 193 proteins with significantly different abundance (p < 0.05).
• Data originates from a novel three-dimensional in vitro model designed to better mimic native tissue architecture.
• Analysis used advanced liquid chromatography-trapped ion mobility spectrometry-tandem mass spectrometry.

Limitations

• Dataset scale is limited to 80.2 KB; row count and column-level documentation are unknown.
• Data is derived from a specific in vitro model; direct applicability to in vivo scenarios requires validation.

Provenance

Source

figshare, author Joel T. Pratt.

Collection Method

Proteomic analysis of small extracellular vesicles isolated via size exclusion chromatography from control and infected cell cultures.

Freshness

Last updated 2026-05-07 05:44:06.