Optimization of Alkaloid Accumulation in Tetradium Daniellii Callus Cultures

A 2026 study by Ji Won Kim details quantitative results from optimizing callus culture conditions for the medicinal plant Tetradium daniellii. The dataset includes measurements of rutaecarpine and evodiamine alkaloid content across different media and light conditions, analyzed via UHPLC-MS/MS. It compares levels in callus cultures, immature fruits, and plantlets to assess sustainable production methods.

Use Cases

• Compare alkaloid accumulation efficiency across different plant tissue culture media (MS, WPM, DKW, SH) based on the experimental conditions described.
• Evaluate the effect of light versus dark conditions on callus induction and secondary metabolite production based on the described incubation protocols.
• Benchmark callus-derived alkaloid yields against traditional sources like immature fruits based on the quantitative comparisons provided.
• Identify optimal tissue types (leaf vs. petiole) for callus culture initiation based on the reported induction rates.

Strengths

• Quantitative results include specific alkaloid concentrations, such as 140.83 mg·kg⁻¹ rutaecarpine in MS medium callus.
• Experimental design is clearly described, comparing multiple culture media, light conditions, and tissue sources.
• Analytical method (UHPLC-MS/MS) is specified, indicating high-confidence measurement of target compounds.

Limitations

• Dataset is small (18.8 KB DOCX file), suggesting limited raw data or a summary report.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale analysis.

Provenance

Source

Ji Won Kim via figshare

Collection Method

Experimental data from in vitro plant tissue culture and UHPLC-MS/MS analysis.

Freshness

Last updated 2026-05-13 05:51:53; freshness should be verified.

Geography

Plant species native to Korea and China; study location unspecified.