Optimized Lipid Extraction Protocol for Individual Mesozooplankton

A methodological paper presents an optimized lipid extraction and annotation workflow for marine zooplankton. The protocol yields a 2.5-fold increase in lipid recovery and a 4.4-fold gain in signal intensity from single Calanus copepods. It was developed by Jiwoon Hwang and published in March 2026.

Use Cases

• Compare lipid annotation performance between the adduct-hierarchy (LOBSTAHS) and fragmentation-based (MS-DIAL) approaches detailed in the paper.
• Apply the described Bligh and Dyer-based extraction protocol with in-line glass bead homogenization to improve lipid recovery from chitinous samples.
• Utilize the integrated wax ester-specific fragmentation library to annotate marine-relevant lipid classes absent from conventional databases.
• Validate the hybrid annotation pipeline for high-resolution, compound-specific lipidomics of individual mesozooplankton.

Strengths

• The protocol is validated to yield a 2.5-fold increase in lipid recovery and a 4.4-fold gain in signal intensity.
• The method is designed for high-resolution lipidomics at the scale of individual mesozooplankton, specifically Calanus copepods.
• The workflow is described as broadly accessible, requiring no additional homogenization equipment or extended extraction steps.

Limitations

• The primary content is a methodological paper (PDF), not a structured dataset with rows and columns for direct computational analysis.
• The 135.2 KB file size indicates the document is a concise paper, not a large-scale data repository.
• Sample lipidomic data or results from applying the protocol are not provided as a separate, machine-readable file.

Provenance

Source

figshare, authored by Jiwoon Hwang.

Collection Method

Developed and validated laboratory protocol for lipid extraction and mass spectrometry analysis.

Freshness

Last updated March 2026.

Geography

Marine ecosystems, with specific application to Calanus copepods.