Antibody CDR3-VH Repertoire Physicochemical Signatures for PS-ASO Recognition

Riccardo Galasso's research dataset contains a shared enriched set of 113 CDR3-VH clonotypes identified through NGS-guided phage display against phosphorothioate-modified antisense oligonucleotides (PS-ASOs). The data reveals a defined physicochemical profile for these antibody fragments, including increased positive charge, constrained length distribution, and recurrent aromatic residue patterns. Representative functional assays, including ELISA and fluorescence microscopy, support the biological relevance of the identified signature.

Use Cases

• Identifying shared physicochemical signatures in antibody CDR3 regions based on clonotype sequence data.
• Training models to predict antibody-oligonucleotide binding using CDR3 charge, pI, and hydrophobicity descriptors.
• Comparing repertoire diversity and clonal dominance between selection rounds in phage display experiments.
• Designing novel antibody binders with tailored properties for phosphorothioate backbone recognition.

Strengths

• Dataset is derived from a reproducible, deep-sequencing guided experimental strategy using Illumina MiSeq.
• Identifies a specific set of 113 enriched CDR3-VH clonotypes with a defined physicochemical profile.
• Data is openly licensed under CC-BY-4.0, facilitating reuse and redistribution.

Limitations

• Column names and exact row counts are not provided, limiting understanding of the data structure.
• The dataset appears to be a single Excel file; the relationship between described analyses and the provided file is unclear.
• Metadata is sparse, lacking information on the organization responsible for the research.

Provenance

Source

Riccardo Galasso

Collection Method

Generated from two independent phage display biopanning strategies coupled with targeted amplicon sequencing and bioinformatic clonotype reconstruction.

Freshness

Last updated on 2026-05-29.