Proteomic Analysis of Lysinibacillus PET Biodegradation Potential

Radoslaw B. Dudziak's dataset contains proteomic and growth assay data from a study evaluating a Lysinibacillus sp. strain isolated from Icelandic soil for PET biodegradation. It compares the strain's early-stage growth performance against the reference Ideonella sakaiensis over a six-week period. The data includes results from proteomic analysis, in silico screening, and surface imaging to assess microbial adaptation to PET.

Use Cases

• Compare early-stage growth assay results between Lysinibacillus sp. and Ideonella sakaiensis over six weeks to identify adaptive advantages.
• Analyze MALDI-TOF MS proteomic data for the expression of oxidative-stress response proteins like peroxiredoxins and superoxide dismutases under PET exposure.
• Correlate FE-SEM surface imaging observations of erosion and delamination with proteomic profiles to infer mechanisms of polymer surface alteration.
• Screen in silico genomic data for the absence of canonical PET-degrading enzyme motifs, such as the GXSXG sequence or Ser-His-Asp catalytic triad.

Strengths

• Data originates from a controlled scientific study with specific comparative growth assays over a defined six-week experimental period.
• Includes multi-method validation combining proteomics (MALDI-TOF MS), in silico screening, and surface imaging (FE-SEM).
• Dataset is openly shared under a CC BY 4.0 license, facilitating reuse and verification.

Limitations

• The dataset is very small (14.4 KB), indicating limited scope, likely containing summary or processed results rather than raw experimental data.
• Proteomic analysis did not detect canonical PET-degrading enzymes, suggesting the data may capture indirect metabolic adaptation rather than direct depolymerization mechanisms.
• Geographic coverage is limited to a single bacterial strain isolated from Icelandic soil, which may not be representative of other environments.

Provenance

Source

Radoslaw B. Dudziak via figshare.

Collection Method

Data generated from laboratory experiments including bacterial isolation, growth assays, proteomic analysis (MALDI-TOF MS), in silico genomic screening, and surface imaging (FE-SEM).

Time Range

Covers a six-week experimental period for growth assays.

Freshness

Last updated on 2026-03-24.

Geography

Bacterial strain isolated from Icelandic soil.