Table 1_Screening and functional validation of key genes in Helicobacter Pylori-induced ma

A 234.7 KB research document by Nengjin Sun, last updated April 2026, analyzes transcriptomic data from H. pylori-infected patients to explore links to migraine and functional dyspepsia. The study identifies 683 differentially expressed genes and four CGRP-related hub genes (PNOC, ICAM1, MMP9, NFE2L1) involved in M1 macrophage polarization. Experimental validation was performed using RT-qPCR and loss/gain-of-function experiments in THP-1 macrophages and SH-SY5Y neuronal cells.

Use Cases

• Identify differentially expressed genes in H. pylori infection based on transcriptomic analysis mentioned in the description
• Investigate immune cell infiltration patterns, particularly M1 macrophage polarization, based on the described immune profiling
• Validate the regulatory relationship between macrophage-derived PNOC and neuronal CGRP expression based on the described co-culture experiments
• Explore functional enrichment of immune and inflammatory pathways based on the GO, KEGG, GSEA, and GSVA analyses described

Strengths

• Identifies 683 differentially expressed genes from H. pylori-infected patient data
• Includes experimental validation via RT-qPCR and functional assays in two cell lines (THP-1 and SH-SY5Y)
• Analysis includes multiple functional enrichment methods (GO, KEGG, GSEA, GSVA) and machine learning approaches

Limitations

• Dataset is a 234.7 KB DOCX file, which is a very small text document, not a primary data repository
• Row and column counts for any underlying data are unknown, limiting suitability assessment
• Column-level documentation for any potential supplementary data is absent; field semantics must be inferred

Provenance

Source

figshare, author Nengjin Sun

Collection Method

Analysis of H. pylori-related datasets from GEO, followed by experimental validation in cell cultures

Freshness

Last updated 2026-04-28 05:30:15