Single-Cell RNA Sequencing of PBMCs from Acral Melanoma Patients on Anti-PD-1 Therapy

Eight acral melanoma patients provided peripheral blood mononuclear cells for single-cell RNA sequencing. The dataset contains profiles of 54,793 high-quality cells, analyzing immune signatures associated with adverse events from anti-PD-1 therapy. Author Qi Bao published the data on figshare in March 2026 under a CC-BY-4.0 license.

Use Cases

• Identify CD8+ T cell differentiation states based on GZMK and GZMB/GNLY/NKG7 expression profiles.
• Calculate the transitional-to-cytotoxic CD8+ T cell ratio as a potential biomarker for irAE risk.
• Analyze enrichment of cell-killing gene pathways in patient groups with and without adverse events.
• Perform pseudotime trajectory analysis on T cell populations from the described patient cohorts.

Strengths

• Data is derived from single-cell RNA sequencing of 54,793 high-quality cells.
• Includes samples from three distinct patient groups: those with irAEs, treated without irAEs, and untreated controls.
• Published under a permissive CC-BY-4.0 license, facilitating reuse and redistribution.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The dataset is small (355.8 KB), derived from only eight patients, limiting statistical power.
• Data may reflect bias inherent to the specific patient cohort and study design on figshare.

Provenance

Source

figshare

Collection Method

Single-cell RNA sequencing performed on peripheral blood mononuclear cells from patient samples.

Time Range

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Freshness

Last updated 2026-03-18 07:44:53

Geography

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