Single-Cell Transcriptomic Profiling of Chicken Immune Response to Salmonella Infection

50 specific-pathogen free chicks were used to study the immune response to Salmonella Enteritidis infection. The dataset likely contains single-cell RNA sequencing and flow cytometry results from ileal intraepithelial lymphocytes collected at 2 and 6 days post-infection. It was authored by Shuja Majeed and last updated on June 3, 2026.

Use Cases

• Identify innate-like cytotoxic T cell populations based on scRNA-seq cluster analysis mentioned in the description
• Analyze transcriptional trajectories of T cell differentiation based on inferred trajectory analysis
• Correlate immune cell proportions with infection status based on flow cytometry and bacteriology results
• Study the role of TCRγδ+CD8αβ+ cytotoxic IELs in early Salmonella defense based on the reported findings

Strengths

• Data integrates results from two complementary techniques: spectral flow cytometry and single-cell RNA sequencing
• Study design includes a control group and an infected group with 25 birds each
• Analysis covers two distinct time points post-infection (2 dpi and 6 dpi)

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• The dataset is very small at 53.1 KB, indicating limited scope

Provenance

Source

figshare

Collection Method

Experimental study combining spectral flow cytometry and single-cell RNA sequencing on chicken ileal samples.

Freshness

Last updated 2026-06-03 04:51:06; freshness should be verified