In Vitro Cefiderocol Resistance in Clinical Escherichia coli Strains

A study document describing the in vitro induction and characterization of cefiderocol resistance in five Escherichia coli strains, including four clinical isolates and the ATCC 25922 reference strain. The research was conducted by Muhammad Ali Syed and the document was last updated on 2026-05-26. The dataset includes findings from whole-genome sequencing, RNA sequencing, and phenotypic assays such as growth kinetics and biofilm formation.

Use Cases

• Identify genetic mutations associated with antibiotic resistance based on whole-genome sequencing results mentioned in the description.
• Analyze changes in gene expression related to iron transport and metabolism using the RNA-Seq data referenced.
• Study phenotypic adaptations like small-colony-variant formation and reduced biofilm production described in the results.
• Investigate the role of specific beta-lactamase enzymes (NDM-5, KPC-2, OXA-48, IMP-4) in cefiderocol resistance.

Strengths

• Includes data from whole-genome sequencing and RNA sequencing for five distinct bacterial strains.
• Phenotypic characterization covers multiple assays: growth kinetics, biofilm formation, and transmission electron microscopy.
• Document is licensed under CC-BY-4.0, permitting open sharing and adaptation.

Limitations

• The primary data file is a 14.7 KB DOCX document, which likely contains a summary report rather than the raw sequencing or assay data.
• Row count and column-level documentation are unknown, limiting assessment of data structure and granularity.
• The dataset's scale is very small (14.7 KB), indicating limited raw data is directly available in this upload.

Provenance

Source

figshare, uploaded by Muhammad Ali Syed.

Collection Method

Data generated via serial passaging of bacterial strains under antibiotic pressure, followed by genomic and phenotypic analysis.

Freshness

Last updated 2026-05-26 06:01:54; freshness should be verified.