In Vivo Efficacy Data for EM86-Loaded Nanofibrous Wound Dressing

Experimental results detail the efficacy of an antimicrobial peptide-loaded nanofibrous dressing against multidrug-resistant Pseudomonas aeruginosa wound infections in a mouse model. The dataset, approximately 120.2 KB in size, was authored by Samar Essam Metwally and uploaded to figshare in April 2026. It contains findings from in vitro and in vivo testing, including minimum inhibitory concentrations and bacterial counts from treated wounds.

Use Cases

• Analyze the correlation between EM86 loading dose (35 ± 18 μg) and reduction in P. aeruginosa SM016 colony counts from mouse wound models.
• Compare the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of EM86 against various multidrug-resistant Gram-negative bacterial isolates.
• Evaluate the relationship between nanofiber scaffold properties (mean diameter of 238 ± 78 nm, swelling capacity of 510%) and treatment efficacy outcomes.
• Assess the time-kill kinetics data showing EM86 killed Pseudomonas aeruginosa SM016 within 60 minutes.
• Study the toxicity profile of EM86 using the IC50 value (>300 μg/mL) from human skin fibroblast (HSF) MTT assays.

Strengths

• Dataset includes specific, quantified experimental results like MIC/MBC values, nanofiber diameter (238 ± 78 nm), and EM86 loading dose (35 ± 18 μg).
• In vivo efficacy is supported by statistically significant results (P < 0.001) comparing bacterial counts in treated versus untreated mouse wounds after 4 days.

Limitations

• The dataset is very small at 120.2 KB, indicating limited scope, likely containing summary results rather than raw experimental data.
• Findings are specific to a single bacterial strain (Pseudomonas aeruginosa SM016) and a BALB/c mouse model, limiting generalizability.

Provenance

Source

Samar Essam Metwally via figshare.

Collection Method

Data generated from laboratory experiments including peptide synthesis, in vitro antimicrobial assays, nanofiber electrospinning, and in vivo mouse wound infection studies.

Time Range

Experimental period not specified; publication date is 2026.

Freshness

Last updated in April 2026.