lnc-FAM164A1-ACLY Axis: Pro-Inflammatory Responses in Human Macrophages

Jian-Guo Wang's research data from 2026 investigates long noncoding RNAs regulating pro-inflammatory activation in human macrophages. The dataset includes results from lncRNA microarray analysis, loss-of-function and gain-of-function experiments, and mouse endotoxemia studies. It identifies 11 LPS-increased lncRNAs and characterizes the lnc-FAM164A1-ACLY interaction promoting NF-κB signaling.

Use Cases

• Identify candidate lncRNAs involved in macrophage activation based on microarray results.
• Study the functional relationship between lnc-FAM164A1 and ACLY protein based on RNA pulldown and immunoprecipitation data.
• Model pro-inflammatory cytokine regulation (CCL2, IL-6, TNF-α) based on silencing and overexpression experiments.
• Investigate NF-κB signaling pathways in human and mouse macrophages based on experimental results.

Strengths

• Includes data from multiple experimental methods: lncRNA microarray, loss/gain-of-function, luciferase assays, RNA pulldown, and mouse models.
• Focuses on primary human macrophages, which may provide more physiologically relevant insights than cell lines.
• Licensed under CC-BY-4.0, permitting open sharing and adaptation.

Limitations

• The dataset is a single 675.2 KB DOCX file, suggesting limited raw data or a focus on summary results.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale computational analysis.

Provenance

Source

figshare, author Jian-Guo Wang

Collection Method

Human lncRNA microarray analysis, functional experiments (silencing, overexpression), luciferase reporter assays, RNA pulldown, RNA immunoprecipitation, and mouse endotoxemia studies.

Freshness

Last updated 2026-05-01 04:19:34; freshness should be verified.