miR-30c-5p/SOCS3 Axis: Inflammation and Metabolism in Duchenne Muscular Dystrophy

A 20.6 KB research document by Yayu Wang details the identification of a key miRNA-mRNA regulatory axis in Duchenne muscular dystrophy. The study integrates bulk RNA-seq, small RNA-seq, and single-cell RNA-seq data from public repositories GSE38417, GSE109178, GSE157668, and GSE213925. Findings suggest miR-30c-5p downregulation and SOCS3 upregulation are associated with inflammatory and metabolic alterations in DMD.

Use Cases

• Validate miRNA-mRNA regulatory networks in muscular dystrophy based on the integrated RNA-seq analysis described.
• Investigate SOCS3 as a hub gene in inflammatory pathways based on its elevated expression in DMD myocytes.
• Model metabolic imbalance in muscle disorders based on the suppressed metabolic processes observed in SOCS3+ cells.
• Benchmark differential expression analysis methods based on the identification of 100 differentially expressed miRNAs.

Strengths

• Integrates data from four distinct public RNA-seq datasets (GSE38417, GSE109178, GSE157668, GSE213925).
• Includes validation experiments in mdx mice, C2C12 myoblasts, and primary skeletal muscle cells.
• Licensed under CC-BY-4.0, facilitating open reuse.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is a 20.6 KB document, indicating limited scope and likely containing summary results rather than raw data.

Provenance

Source

figshare

Collection Method

Integrated analysis of public RNA-seq datasets and experimental validation.

Freshness

Last updated 2026-05-22 05:35:33; freshness should be verified.