Sm29 Antigen Transcriptomic Modulation Across Leprosy Reactional Forms

Transcriptome data from peripheral blood mononuclear cells stimulated with the Schistosoma mansoni Sm29 antigen and Mycobacterium leprae antigen. The dataset, authored by Joyce Karoline Silva and last updated in June 2026, likely contains results from differential expression analysis using DESeq2 and pathway enrichment analysis via Ingenuity Pathway Analysis. It focuses on comparing immune responses across leprosy clinical forms: reversal reaction, erythema nodosum leprosum, and without reaction.

Use Cases

• Identify differentially expressed genes based on stimulation with the rSm29 antigen versus M. leprae antigen.
• Analyze enriched immune pathways, such as those involving CSF2, IL10, IFN-α, and chemokines, across different leprosy reactional states.
• Compare transcriptomic regulatory landscapes between reversal reaction and erythema nodosum leprosum patient samples.
• Investigate the potential of the Sm29 antigen as an immunomodulator for managing leprosy reactional episodes.

Strengths

• Data is derived from RNA sequencing performed on an Illumina Nextseq 2000 platform with 2x100 bp reads, suggesting high-quality sequencing.
• Analysis includes differential expression with DESeq2 and pathway enrichment with Ingenuity Pathway Analysis, indicating a structured bioinformatics workflow.
• Dataset is licensed under CC-BY-4.0, allowing for open sharing and reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (280.9 KB), indicating limited scope, likely containing summary results rather than raw sequence data.

Provenance

Source

figshare

Collection Method

Peripheral blood mononuclear cells were cultured and stimulated, followed by RNA extraction, library preparation, and sequencing.

Freshness

Last updated 2026-06-01 04:17:38; freshness should be verified.