Table 1_Toward precision detection of pyrazinamide resistance: critical concentration asse

110 rifampicin-resistant tuberculosis isolates from Beijing were tested for pyrazinamide resistance using broth microdilution at two concentrations and a fluorescence PCR assay. Broth microdilution at 200 μg/mL showed 92.2% agreement with whole-genome sequencing for mutant isolates and 94.9% for wild-type. Yanfeng Zhao published this data on figshare in May 2026.

Use Cases

• Compare phenotypic and genotypic resistance detection methods based on broth microdilution and whole-genome sequencing results.
• Assess the performance of the MeltPro MTB/PZA assay for rapid detection of pncA mutations.
• Evaluate the impact of critical concentration selection on resistance rate reporting based on the 100 μg/mL and 200 μg/mL comparison.

Strengths

• Dataset includes 110 clinical isolates, providing a substantive sample size for analysis.
• Validation uses whole-genome sequencing as a genotypic comparator, a high-resolution reference method.
• Results are disaggregated by two different critical concentrations (100 μg/mL and 200 μg/mL).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect geographic bias inherent to its collection from Beijing institutions.

Provenance

Source

Yanfeng Zhao via figshare.

Collection Method

Isolates were collected from TB prevention and control institutions and designated hospitals in Beijing. Susceptibility was tested via broth microdilution and fluorescence PCR melting curve analysis.

Freshness

Last updated 2026-05-25 05:31:07; freshness should be verified.

Geography

Beijing, China.